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OJBTM
Online Journal of Bioinformatics ©
Volume 14 (1): 66-75, 2013
Identification of single nucleotide polymophisms
using CLC and SeqMan transcriptome sequencing data.
Manisha R. Sajnani, Vaibhav
D. Bhatt and Chaitanya G. Joshi*
Department of Animal Biotechnology, College of
Veterinary Science and Animal Husbandry, Anand Agricultural University, Gujarat, India.
ABSTRACT
Sajnani MR,
Bhatt VD, Joshi CG., Identification of single nucleotide polymophisms
using CLC and SeqMan transcriptome sequencing data,
Online J Bioinform., 14 (1):
66-75, 2013. Next generation
sequencing (NGS) technologies produce very large amount of data at low cost. Single
Nucleotide Polymorphisms (SNPs) are the most abundant form of genetic
variation and need to be identified
quickly. The performance of CLC and SeqMan for prediction of potential SNPs from human buccal
cancer and healthy transcriptome data obtained from Roche 454 sequencing
technology based on software utility, time, memory, disk space and accuracy are
described.
Keywords: Sequencing,
Single Nucleotide Polymorphisms, CLC, SeqMan, Buccal,
Transcriptome
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