©1996-2019 All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Bioinformatics©
Volume 8 (1):115-138, 2007
In-silico TAT-PTD prediction for cell penetrating peptides.
Tandon C, Aggarwal A, Goel P, Sengupta D, Naik P
of Biotechnology and Bioinformatics,
Tandon C, Aggarwal A, Goel P, Sengupta D, Naik P., In-silico TAT-PTD prediction for cell penetrating peptides, Onl J Bioinform, 8 (1):115-138, 2007. Cell Penetrating Peptides (CPP) enhance transport of membrane and secretory proteins across hydrophobic membranes. Natural or synthetic CPP water soluble peptides translocate across cell membranes and are used for intracellular delivery of large hydrophilic molecules. HIV-TAT PTD with an amino acid sequence of YGRKKRRQRRR is the core of TAT protein in HIV, with 8 of its 11 TAT-PTD amino acids being hydrophilic yet this molecule diffuses readily through hydrophobic bi-lipid membranes. The effect of charged amino-acids in TAT-PTD on membrane transport was therefore evaluated In silico. A dataset of 168 sequences was created mutating the original TAT-PTD 11-amino acid sequence. Qikprop was used to predict ADME properties and a correlation of R2 = 73.7% between partition coefficient and skin permeability was found.
Keywords: Partition Coefficient, Skin Permeability, TAT- PTD, Cell Penetrating Peptides, Homology modeling.