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OJBTM

 Online Journal of Bioinformatics 

Volume 12(2):255-273, 2011


Phylogenetic analysis of mitochondrial 16s rRNA and NADH Dehydrogenase genes

of Takifugu (Pisces: Tetraodontiformes: Tetraodontidae)

 

M. Jagannath (MSc), K. Umadevi (PhD) * and Subhalaxmi Nayak (MSc)

 

DBT BIF Programme, Department of Marine Living Resources, Andhra University, Visakhapatnam, India,

 

ABSTRACT

 

 Jagannath M, Umadevi K, Subhalaxmi N., Phylogenetic analysis of mitochondrial 16s rRNA and NADH Dehydrogenase genes of Takifugu (Pisces: Tetraodontiformes: Tetraodontidae), Online J Bioinformatics, 12(2):255-273, 2011. Phylogenetic analysis of mitochondrial 16s rRNA and NADH Dehydrogenase (ND) gene sequences of Takifugu (Pisces: Tetraodontiformes: Tetraodontidae) was performed.   Mitochondrial 16s rRNA genes and orthologous mitochondrial ND gene subunits sequences were extracted from GenBank and multiple sequence alignment was performed using ClustalX. The phylogenetic tree was constructed by maximum parsimony with PHYLIP (Phylogeny Inference Package).  Results showed that  ND IV gene was the primitive ancestor in Takifugu genus. Each group of the phylogram  showed  +90%  identity suggesting that the sequences are highly conserved.  Takifugu snyderi and Takifugu stictonotus, Takifugu rubripes and Takifugu chinensis, Takifugu obscurus and Takifugu fasciatus, Takifugu niphobles and Takifugu pardalis, Takifugu poecilonotus and Takifugu exascurus were closely associated

 

Keywords: Phylogeny, PHYLIP, 16s rRNA, NADH Dehydrogenase, Takifugu.


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