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OJBTM
Online Journal of Bioinformatics ©
Volume 12(2):255-273, 2011
Phylogenetic
analysis of mitochondrial 16s rRNA and NADH
Dehydrogenase genes
of Takifugu
(Pisces: Tetraodontiformes: Tetraodontidae)
M. Jagannath (MSc), K. Umadevi (PhD)
* and Subhalaxmi Nayak
(MSc)
DBT – BIF Programme, Department of Marine Living Resources, Andhra
University, Visakhapatnam, India,
ABSTRACT
Jagannath M, Umadevi K, Subhalaxmi N., Phylogenetic analysis of mitochondrial 16s rRNA and NADH Dehydrogenase genes of Takifugu
(Pisces: Tetraodontiformes: Tetraodontidae),
Online J Bioinformatics,
12(2):255-273, 2011. Phylogenetic analysis of mitochondrial 16s rRNA and NADH Dehydrogenase (ND) gene sequences of Takifugu (Pisces: Tetraodontiformes:
Tetraodontidae) was performed.
Mitochondrial 16s rRNA genes and orthologous
mitochondrial ND gene subunits sequences were extracted from GenBank and multiple sequence alignment was performed using
ClustalX. The phylogenetic tree was constructed by
maximum parsimony with PHYLIP (Phylogeny Inference Package). Results
showed that ND IV gene was the primitive
ancestor in Takifugu genus. Each group of the phylogram showed
+90% identity suggesting that the sequences are highly conserved. Takifugu snyderi
and Takifugu stictonotus,
Takifugu rubripes
and Takifugu chinensis,
Takifugu obscurus and Takifugu fasciatus,
Takifugu niphobles
and Takifugu pardalis,
Takifugu poecilonotus
and Takifugu exascurus
were closely associated
Keywords:
Phylogeny, PHYLIP, 16s rRNA, NADH Dehydrogenase, Takifugu.
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