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Online
Journal of Bioinformatics©
Onl J Bioinform©
Established 1995
ISSN 1443-2250
Volume 25 (3) :147-158, 2024
In silico cis-regulation
of seed protein promoters in wheat, rice and oat clones.
Yadav D, Singh VK, Singh NK
College of Basic Sciences and Humanities G.B. pant University of
Agriculture and Technology, Pantnagar, National
Research Center on Plant Biotechnology Indian Agriculture Research Institute,
New Delhi
ABSTRACT
Yadav D,
Singh VK, Singh NK., In silico cis-regulation of seed protein promoters in
wheat, rice and oat clones, Onl J Bioinform.,
25 (3) :147-158, 2024. A total of 24
promoter sequences with assigned accession number EF393165 to EF393188 and
representing major seed storage proteins of wheat namely High molecular weight glutenin subunit (HMW-GS), low molecular weight glutenin subunits (LMW-GS) alpha/beta gliadins, triticin along with rice glutelins
and oat 12S globulins were cloned from indigenous cultivars of wheat, rice and
oat and was subjected to in silico analysis using bioinformatic
softwares for the presence of different cis-regulatory motifs. The phylogeny
studies based on the multiple sequence alignment of these promoters revealed
four distinct clusters showing major group of seed storage promoters. The
presence of additional motifs like RY repeats, ABRE, AC-11, CAAT box, LTR, UTR,
CCGTCC box, G box, GARE, MBS along with the common motifs present in seed
storage promoters like Prolamin-box, TATA, CAAT
provides a better option for multifarious uses.
Keywords: Seed storage
protein promoters, Cis-regulatory Elements, In silico.
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