©1996-2019 All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Bioinformatics©
Volume 8 (1):75-83, 2007
In silico Cis-regulatory Elements Analysis of Seed
Storage Protein Promoters Cloned
from Different Cultivars of Wheat, Rice and Oat
Yadav D1, Singh VK1, Singh NK2
1Department of Molecular Biology and Genetic
Yadav D, Singh VK, Singh NK., In silico Cis-regulatory Elements Analysis of Seed Storage Protein Promoters Cloned from Different Cultivars of Wheat, Rice and Oat, Onl J Bioinform, 8 (1):75-83, 2007. A total of 24 promoter sequences with assigned accession number EF393165 to EF393188 and representing major seed storage proteins of wheat namely High molecular weight glutenin subunit (HMW-GS), low molecular weight glutenin subunits (LMW-GS) alpha/beta gliadins, triticin along with rice glutelins and oat 12S globulins were cloned from indigenous cultivars of wheat, rice and oat and was subjected to In silico analysis for presence of different cis-regulatory motifs. The phylogeny studies based on the multiple sequence alignment of these promoters revealed four distinct clusters showing major group of seed storage promoters. The presence of additional motifs like RY repeats, ABRE, AC-11, CAAT box, LTR, UTR, CCGTCC box, G box, GARE, MBS along with the common motifs present in seed storage promoters like Prolamin-box, TATA, CAAT provides a better option for multifarious uses.
Keywords: Seed storage protein promoters, Cis-regulatory Elements, In silico.