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OJBTM

Online Journal of Bioinformatics

 

Volume 8 (1):75-83, 2007


In silico Cis-regulatory Elements Analysis of Seed Storage Protein Promoters Cloned
from Different Cultivars of Wheat, Rice and Oat

 

Yadav D1,  Singh VK1, Singh NK2

 

1Department of Molecular Biology and Genetic Engineering, College of Basic Sciences and Humanities G.B. pant University of Agriculture and Technology, Pantnagar (Uttarakhand) 2National Research Center on Plant Biotechnology Indian Agriculture Research Institute, New Delhi 110012

 

ABSTRACT

 

Yadav D,  Singh VK, Singh NK., In silico Cis-regulatory Elements Analysis of Seed Storage Protein Promoters Cloned from Different Cultivars of Wheat, Rice and Oat, Onl J Bioinform,  8 (1):75-83, 2007. A total of 24 promoter sequences with assigned accession number EF393165 to EF393188 and representing major seed storage proteins of wheat namely High molecular weight glutenin subunit (HMW-GS), low molecular weight glutenin subunits (LMW-GS) alpha/beta gliadins, triticin along with rice glutelins and oat 12S globulins were cloned from indigenous cultivars of wheat, rice and oat and was subjected to In silico analysis for presence of different cis-regulatory motifs. The phylogeny studies based on the multiple sequence alignment of these promoters revealed four distinct clusters showing major group of seed storage promoters. The presence of additional motifs like RY repeats, ABRE, AC-11, CAAT box, LTR, UTR, CCGTCC box, G box, GARE, MBS along with the common motifs present in seed storage promoters like Prolamin-box, TATA, CAAT provides a better option for multifarious uses.

 

Keywords: Seed storage protein promoters, Cis-regulatory Elements, In silico.


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