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Online
Journal of Bioinformatics©
Onl J Bioinform©
Established 1995
ISSN 1443-2250
Volume 25 (3) :113-118,
2024.
Growth promoter for Porteresia coarctata crop
Mahalakshmi S, Krishna NC,
Rao KV, Reddy VD.
Centre
for Plant Molecular Biology, Osmania University, Hyderabad, India
ABSTRACT
Mahalakshmi S,Krishna NC,Rao KV,Reddy VD Growth promoter
for Porteresia coarctata crop, Onl J Bioinform 25 (3) :119-129, 2024. In
silico identification of promoters is complicated by the hierarchical, modular
and diverse nature of their architecture. Tools developed for prediction of
eukaryote promoters produce acceptable results for certain species but are not
as specific for plants. An algorithm to predict plant-promoters is described.
The program was trained on 255 plant promoters and verified with 50 promoters.
The tool was able to accurately identify 72% of the test sequences. A promoter
sequence designated as Pccp was identified from the
genomic sequences of Porteresia coarctata. The
tool was validated with laboratory results. Cloning of Pccp
upstream to gusA and introduction of the fusion-construct
into plant tissues revealed GUS expression confirming predicted sequence as the
promoter
Key words: Porteresia coarctata, promoter,
isolation, fusion construct .