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Online Journal of Bioinformatics

Volume 13(1):22-28, 2012

In silico analysis of bacterial DNA polymerases and molecular phylogeny


Meghashri Sridhar, Ashwini Manjunath, Jyothi Bala Chauhan, Farhan Zameer*


Department of Biotechnology and Microbiology, Mahajana Life Science Research Centre, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, K.R.S Road, Metagalli, Mysore,. Karnataka, India.




Sridhar M, Manjunath A, Bala Chauhan J, Zameer F., In silico analysis of bacterial DNA polymerases and molecular phylogeny, Online J Bioinform, 13(1):22-28, 2012. PCR and probe based nucleic acid detection are used increasingly in diagnostics. PSI-BLAST (screening), Clustal W (sequence homology), phylogenic analysis and structure prediction were used to show that Taq polymerase has sequence similarity with many of the other bacterial polymerase sharing structural domains XPG, DNA polyA and Taqexonuc superfamilies. The findings suggested that Taq polymerase might be replaced and diagnosis carried out at isothermal conditions.


Keywords: In silico, Bacterial Polymerase, Domain, Phylogeny, PCR