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Volume
13(1):22-28, 2012
In
silico analysis of bacterial DNA polymerases and molecular phylogeny
Meghashri Sridhar, Ashwini Manjunath, Jyothi Bala Chauhan, Farhan Zameer*
Department of Biotechnology and Microbiology, Mahajana
Life Science Research Centre, Pooja Bhagavat Memorial
Mahajana Post Graduate Centre, K.R.S Road, Metagalli, Mysore,. Karnataka,
India.
ABSTRACT
Sridhar
M, Manjunath A, Bala
Chauhan J, Zameer F., In silico analysis of bacterial
DNA polymerases and molecular phylogeny, Online J Bioinform,
13(1):22-28, 2012. PCR and probe based nucleic acid detection are used increasingly
in diagnostics. PSI-BLAST (screening), Clustal W
(sequence homology), phylogenic analysis and structure prediction were used to
show that Taq polymerase has sequence similarity with
many of the other bacterial polymerase sharing structural domains XPG, DNA polyA and Taqexonuc superfamilies. The findings suggested that Taq polymerase might be replaced and diagnosis carried out
at isothermal conditions.
Keywords:
In
silico,
Bacterial Polymerase, Domain, Phylogeny, PCR