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Online Journal of Bioinformatics ©
Volume 20(2):124-134, 2019.
Identification of potential drug-targets in human pathogen Clostridium perfringens
Gagan Chabra, Pramila Sharma, Avishek Anant, Sachin Deshmukh, Himani Kaushik,
Keshav Gopal, Nutan Srivastava, Neeraj Sharma and Lalit C. Garg.
Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi – 110067, India
Chabra C, Sharma P, Anant A, Deshmukh S, Kaushik H, Gopal K, Srivastava N, Sharma N, Garg LC., Identification of potential drug-targets in human pathogen Clostridium perfringens, Onl J Bioinform., 20(2):124-134, 2019. Authors report comparative analysis of human and Clostridium perfringens genomes to identify genes essential for pathogen survival in non-homologous human host for potential drug targets. We found 426 C perfringens gene potential drug targets which were significantly lower than the genome’s protein coding capacity of 2558. The genes were analyzed for overall similarities with essential genes of 14 bacteria in Database of Essential Genes (DEG). Our results showed only 5 essential genes of C. perfringens that exhibited similarity with 12 bacteria species of 14 in DEG database. Of these, 1 gene was similar in 12 species and 4 in 11 species. Thus, the study opens a new avenue for development of potential drugs against highly pathogenic bacterium. Further, by selecting these essential genes of C. perfringens, which are common and essential for other pathogenic microbial species, a broad spectrum anti-microbial drug can be developed. As a case study, we have built a homology model of one of the potential drug targets, ABC transporter-ATP binding protein, which can be employed for in silico docking studies for suitable inhibitors.
Keywords: Clostridium perfringens, DEG, Essential genes, Drug targets, Broad-spectrum antimicrobials.