©1996-2021
All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in
any form except for your own personal use. All other usage or distribution is
illegal under international copyright treaties. Permission to
use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is exclusively
copyrighted in its entirety to OJB publications. This article may be copied
once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing
requirements (DEST) for the Higher Education Research Data Collection
(Australia). Linking:To link to this page or any
pages linking to this page you must link directly to this page only here rather
than put up your own page.
OJBTM
Online Journal of Bioinformatics ©
Volume 20(2):124-134, 2019.
Identification of potential drug-targets in human pathogen Clostridium
perfringens
Gagan
Chabra, Pramila Sharma, Avishek Anant, Sachin Deshmukh, Himani Kaushik,
Keshav
Gopal, Nutan Srivastava, Neeraj
Sharma and Lalit C. Garg.
Gene Regulation Laboratory, National
Institute of Immunology, Aruna Asaf
Ali Marg, New Delhi – 110067, India
ABSTRACT
Chabra
C, Sharma P, Anant A, Deshmukh
S, Kaushik H, Gopal K, Srivastava N, Sharma N, Garg LC., Identification of potential drug-targets in
human pathogen Clostridium perfringens,
Onl J Bioinform., 20(2):124-134, 2019. Authors report
comparative analysis of human and Clostridium perfringens genomes to identify
genes essential for pathogen survival in non-homologous human host for potential
drug targets. We found 426 C perfringens
gene potential drug targets which were significantly lower than the genome’s
protein coding capacity of 2558. The genes were analyzed for overall similarities
with essential genes of 14 bacteria in Database of Essential Genes (DEG). Our
results showed only 5 essential genes of C. perfringens that exhibited
similarity with 12 bacteria species of 14 in DEG database. Of these, 1 gene was
similar in 12 species and 4 in 11 species. Thus, the study opens a new avenue
for development of potential drugs against highly pathogenic bacterium.
Further, by selecting these essential genes of C. perfringens, which are
common and essential for other pathogenic microbial species, a broad spectrum
anti-microbial drug can be developed. As a case study, we have built a homology
model of one of the potential drug targets, ABC transporter-ATP binding
protein, which can be employed for in silico docking studies for suitable
inhibitors.
Keywords: Clostridium perfringens, DEG,
Essential genes, Drug targets, Broad-spectrum antimicrobials.
FULL-TEXT (SUBSCRIBE OR
PURCHASE TITLE)