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OJBTM
Online
Journal of Bioinformatics©
Volume 21(1): 21-37, 2020.
Identification of potential drug-targets in human pathogenic
Clostridium perfringens SM101.
Gagan
Chhabra, Pramila Sharma, Avishek
Anant, Sachin Deshmukh, Himani Kaushik,
Keshav
Gopal, Nutan Srivastava, Neeraj
Sharma and Lalit C. Garg†
Gene Regulation Laboratory, National
Institute of Immunology, Aruna Asaf
Ali Marg, New Delhi – 110067, India
ABSTRACT
Chabra
G, Sharma P, Anant A, Deshmukh
S, Kaushik H, Gopal K, Srivastava N, Sharma N, Garg LC., Identification of potential drug-targets in
human pathogen Clostridium perfringens
SM101, Onl J Bioinform.,
Volume 21(1): 21-37,
2020. We describe comparative genome analysis of Clostridium
perfringens with human genome to identify genes essential for the
bacteria’s survival in non-homologous human host to identify potential drug
targets. We found only 426 gene potential drug targets compared with 2558
genome’s protein coding genes. The 426 genes were further analyzed for
similarity with essential genes of 14 different bacterial species present in
Database of Essential Genes (DEG). We found only 5 essential genes of C.
perfringens similar to those in DEG. Of these, 1 gene was similar in 12 species
and 4 genes similar in 11 species. Thus, the study opens a new avenue for development
of broad spectrum antibiotic against highly pathogenic bacterium by selecting
these essential common genes in pathogenic microbial species. As a case study,
we constructed a homology model drug target, ABC transporter-ATP binding
protein, for in silico docking.
Keywords: Clostridium perfringens, DEG,
Essential genes, Drug targets, Broad-spectrum antimicrobial drug.
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