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Online Journal of Bioinformatics©
Volume 21(1): 21-37, 2020.
Identification of potential drug-targets in human pathogenic Clostridium perfringens SM101.
Gagan Chhabra, Pramila Sharma, Avishek Anant, Sachin Deshmukh, Himani Kaushik,
Keshav Gopal, Nutan Srivastava, Neeraj Sharma and Lalit C. Garg†
Gene Regulation Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi – 110067, India
Chabra G, Sharma P, Anant A, Deshmukh S, Kaushik H, Gopal K, Srivastava N, Sharma N, Garg LC., Identification of potential drug-targets in human pathogen Clostridium perfringens SM101, Onl J Bioinform., Volume 21(1): 21-37, 2020. We describe comparative genome analysis of Clostridium perfringens with human genome to identify genes essential for the bacteria’s survival in non-homologous human host to identify potential drug targets. We found only 426 gene potential drug targets compared with 2558 genome’s protein coding genes. The 426 genes were further analyzed for similarity with essential genes of 14 different bacterial species present in Database of Essential Genes (DEG). We found only 5 essential genes of C. perfringens similar to those in DEG. Of these, 1 gene was similar in 12 species and 4 genes similar in 11 species. Thus, the study opens a new avenue for development of broad spectrum antibiotic against highly pathogenic bacterium by selecting these essential common genes in pathogenic microbial species. As a case study, we constructed a homology model drug target, ABC transporter-ATP binding protein, for in silico docking.
Keywords: Clostridium perfringens, DEG, Essential genes, Drug targets, Broad-spectrum antimicrobial drug.