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Online Journal of Bioinformatics ©
Volume 12(1):18-33, 2011
Comparative evolutionary analysis of metallo-β-lactamases with reference to NDM1
Odedra D1 (M.Sc), Mallick M1 (M.Sc), Vidyarthi AS1 (Ph.D), Shankaracharya1* (M.Sc)
Department of Biotechnology, Birla Institute of Technology, Mesra, Ranchi
Odedra D, Mallick M, Vidyarthi AS, Shankaracharya D, Comparative evolutionary analysis of metallo-β-lactamases with reference to NDM1, Onl J Bioinform., 12(1):18-33, 2011. The amino acid sequences of Metallo-β-lactamases in fast evolving carbapenem resistant E. coli and K. pneumoniae were used for addressing demographic relationships on an evolutionary scale. The study was undertaken to establish the relation of New Delhi metallo-β-lactamase (NDM1) with various other types of metallo-β-lactamases. Results demonstrated well resolved topologies of trees indicating separate line of evolution of NDM1 from other types of metallo-β-lactamases. This was the first systematic study to establish a substitution rate as high as 0.0005 substitutions per site per year for NDM1. Structural alignment of Metallo-β-lactamases from various bacterial strains revealed a highly conserved zinc binding motif His116-X-His118-X-Asp120. This estimate fairly mirrored the quick and regular emergence of new variant forms of metallo-β-lactamases. However, none of the trees could demonstrate geographical separation suggesting the spread of bacterial isolates due to geographical migration of patients for seeking medical services.
Keywords: metallo-β-lactamases, NDM1, carbapenem resistance, evolution