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OJB©
Online Journal of Bioinformatics©
Volume 6
(2):74-90, 2005.
In silico identification
and modelling of a putative iron-regulated TonB-dependant
outer membrane
receptor
protein from the genome of Leptospira
interrogans serovar
Lai.
Sritharan M, Ramadevi S, Pasupala N, Asuthkar S, Tajne S.
Department of Animal Sciences,
Sritharan M, Ramadevi S, Pasupala N, Asuthkar S, Tajne S., In
silico identification and modelling of a putative iron-regulated TonB-dependant outer membrane receptor protein from the
genome of Leptospira interrogans
Serovar Lai, Onl J Bioinform., 6 (2):74-90, 2005. Identification
and modelling of a leptospiral TonB-dependant
outer membrane protein homologous to Fe3+-enterobactin
receptor FepA of Escherichia coli is described. This protein
LEP_IRMP (leptospiral iron-regulated
membrane protein),
has a fold of 3
domains: the β-barrel, plug domain and N-terminal TonB
box, characteristic
of bacterial outer membrane receptors involved in the transport of iron. The
Fur box was identified upstream of the lep_irmp gene encoding this protein, implying
the possible regulation of its expression by iron. Also, the genes for the Fur
regulator and heme oxygenase are found as
neighbourhood genes. The FRAP-NPNL motif involved in heme
binding is present in LEP_IRMP. Using sequence-specific primers, the
full-length lep irmp gene
in several pathogenic Leptospira sp.
and absence in non-pathogenic leptospires has been identified.
The possible role of the protein in heme binding by
pathogenic leptospires is discussed in the light of
the above observations as
well as the failure to detect siderophores
under conditions of iron deprivation.
KEY WORDS: iron,
TonB-dependant outer membrane protein, LEP_IRMP, Leptospira, plug domain, β-barrel domain,
N-terminal TonB box, heme
binding.