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OJBTM
Online
Journal of Bioinformatics ©
Volume 16 (2): 129-143, 2015.
Prioritizing drug targets
in metabolic pathway of Laribacter hongkongensis
Iftikhar
Aslam Tayubi
Faculty of Computing and Information
Technology, King Abdulaziz University, Rabigh, Saudi Arabia.
ABSTRACT
Aslam Tayubi
I., Prioritizing drug targets in metabolic pathway of Laribacter
hongkongensis, Onl J Bioinform., 16 (2): 129-143, 2015. The availability of complete genome sequences
of pathogenic bacteria accelerates the process of drug target identification.
Further, the emergence of rapidly mutating strains evokes the necessity for the
discovery of new drug targets. Hence,
the objective of this study is to determine the highly expressed genes
responsible in cellular pathways of Laribacter hongkongensis, where pyrimidine biosynthesis was our major
pathway of interest. Laribacter
hongkongensis
is a gram-negative, seagull- or spiral rod-shaped potentially bacteria with
global pathogenic occurrence causing diseases such as the community-acquired
Gastroenteritis and Traveller’s Diarrhoea.
The highly expressed genes in related biological pathways were determined, from
which the pseudogenes, hypothetical proteins and
enzymes common to
human species were
systematically filtered out and eliminated. From the remaining enzymes,
2 key enzymes were selected based on their position of occurrence in the
metabolic pathways. Subsequently, the 3- dimensional model of key enzyme
Dihydroorotase from pyrimidine biosynthesis was predicted using MODELLER and
validated using SAVS. The interaction of Dihydroorotase enzyme with three
inhibitors was assessed by GOLD software. The study resulted in shortlisting of
the highly expressed genes specific to the pathway of interest and in addition,
resulted in determining the conserved residues at the binding site of DHO which
can be targeted for designing inhibitor molecules, comprising a comprehensive
approach which can be further, applied to other pathogens of clinical
importance.
Key-Words:
In Silico, Drug Target, Metabolic-Pathway, Laribacter.
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