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Online Journal of Bioinformatics ©

Established 1995

ISSN  1443-2250


Volume 24 (2):65-73, 2023.

In silico Staphylococcus aureus vaccine epitopes.


Garima Bajetha, Sarika Anil Rai.


Agricultural Bioinformatics, Indian Agriculture Statistics Research Institute (I.C.A.R) Library Avenue, Pusa Campus New Delhi- India.




Bajetha G, Anil Rai S., In silico Staphylococcus aureus vaccine epitopes, Onl J Bioinform, 24 (2):65-73, 2023. Staphylococcus aureus adherence to extracellular matrix and plasma proteins enhances infections by orthopaedic-devices.  We report in silico peptide vaccine without adhesion protein sequence, structure, QSAR and protein fold for B- and T-cell epitopes. We found IRKLGVGIA from Immunoglobulin G-binding protein A, IEFLEVVGL from Conserved virulence factor B IISLGALAVASS from Staphylocoagulase and LLVIRTKGTIA from Alpha-Hemolysin] to induce antigenicity with potential to bind HLA alleles DRB1*0101, and DRB1*0401).  In silico exo-membrane antigenicity ranged for staphylocoagulase 0.8080 to conserved virulence factor B only 0.4189. For amino acid (aa) topology, conserved virulence factor B 1-300aa, staphylocoagulase 1-658aa and alpha-hemolysin 1-319aa were fully exposed to outer membrane. Exo-membrane for immunoglobulin G-binding protein A was 35-450aa. MHC Class-I binding scores for protective epitope antigens at 4% threshold with Staphylocoagulase 2.4058, 2.3893 for immunoglobulin G-binding protein A, 2.3246 for alpha-hemolysin and 1.3765 for conserved virulence factor B. For quantitative structure–activity relationship, IC50 and antigenicity we selected DRB1*0101 and DRB1*0401 TTHADGTAT from Staphylocoagulase  for vaccine.


Key words: - Staphylococcus aureus, Epitope, Mastitis, vaccine designing, Immune response.