©1996-2019. All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Bioinformatics ©
Volume 18(2):68-77, 2017.
In silico prediction for Staphylococcus aureus vaccine epitopes.
Garima Bajetha, Sarika and Anil Rai
Center of Agricultural Bioinformatics, Indian Agriculture Statistics Research Institute (I.C.A.R) Library Avenue, Pusa Campus New Delhi-110012 INDIA
Garima Bajetha S, Anil Rai., In silico prediction for Staphylococcus aureus vaccine epitopes. Onl J Bioinform., 18(2):68-77, 2017. Staphylococcus aureus adheres to extracellular matrix and plasma proteins on orthopaedic-devices causing mastitis. To develop a peptide vaccine against the pathogen, a method to extract adhesins from the cell wall of S. aureus cell is desirable. We use In silico structure, QSAR, and simulation with fold level analysis to predict potential antigenic B-cell epitope derived T-cell epitopes from four vaccine targets. The models showed accuracy of 70-97%. VaxiJen analysis of exo-membrane full length proteins exhibited antigenicity ranging 0.4189 to 0.8080. Of 4 essential membrane proteins, staphylocoagulase “coa” showed highest antigenicity. Based on antigenicity, cluster scores, IC50 value, homology and stimulation results “TTHADGTAT” from Staphylocoagulase “coa” was most suitable for vaccine. Selected epitopes demonstrate potential to induce B-cell and T-cell mediated immune responses.
Key words: - Staphylococcus aureus, Epitope, Mastitis, vaccine designing, Immune response.
FULL-TEXT (SUBSCRIPTION OR PURCHASE TITLE $25USD)