©1996-2019. All Rights Reserved. Online Journal of Bioinformatics . You
may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties.
Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from
the publisher. This article is exclusively copyrighted in its entirety to OJB
publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the
editors. This journal satisfies the refereeing requirements (DEST) for the Higher
Education Research Data Collection (Australia). Linking: To link to this page or any pages
linking to this page you must link directly to this page only here rather than
put up your own page.
OJBTM
Online Journal of Bioinformatics ©
Volume 18(2):68-77, 2017.
In silico prediction for Staphylococcus aureus vaccine epitopes.
Garima Bajetha, Sarika
and Anil Rai
Center
of Agricultural Bioinformatics, Indian Agriculture Statistics Research
Institute (I.C.A.R) Library Avenue, Pusa Campus New
Delhi-110012 INDIA
ABSTRACT
Garima Bajetha S, Anil Rai., In silico prediction for Staphylococcus aureus vaccine
epitopes. Onl J Bioinform.,
18(2):68-77,
2017. Staphylococcus
aureus adheres to extracellular matrix and
plasma proteins on orthopaedic-devices causing mastitis. To develop a peptide
vaccine against the pathogen, a method to extract adhesins
from the cell wall of S. aureus cell
is desirable. We use In silico structure, QSAR, and simulation
with fold level analysis to predict potential antigenic B-cell epitope derived
T-cell epitopes from four vaccine targets. The models showed accuracy of
70-97%. VaxiJen analysis of exo-membrane
full length proteins exhibited antigenicity ranging 0.4189 to 0.8080. Of 4
essential membrane proteins, staphylocoagulase “coa” showed highest antigenicity. Based on antigenicity,
cluster scores, IC50 value, homology and stimulation results
“TTHADGTAT” from Staphylocoagulase “coa” was most suitable for vaccine. Selected epitopes
demonstrate potential to induce B-cell and T-cell mediated immune responses.
Key words: - Staphylococcus aureus, Epitope,
Mastitis, vaccine
designing, Immune response.
FULL-TEXT (SUBSCRIPTION OR PURCHASE TITLE
$25USD)