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OJBTM
Online Journal of Bioinformatics ©
Volume
12(2):323-328, 2011
Genetic
diversity of hatchery stocks of Macrobrachium
rosenbergii assessed by microsatellite DNA.
E. Suresh, A.K.
Reddy, Gopal Krishna, Aparna Chaudhari,
Rupam Sharma and P. Gireesh
Babu
1 Division of Fish Genetics and Biotechnology,
Central Institute of Fisheries Education, Mumbai-400 061, India. Correspondence: PhD Scholar, Division of
Fish Genetics and Biotechnology, Central Institute of Fisheries Education,
Indian Council of Agricultural Research, Off Yari
Road, Versova, Mumbai - 400 061, India.
ABSTRACT
Suresh
E, Reddy AK, Krishna G, Chaudhari A, Sharma R, Babu PG., Genetic diversity of hatchery stocks of Macrobrachiumrosenbergii assessed by microsatellite DNA, Onl J Bioinform., 12(2):323-328,
2011. Five microsatellite DNA loci were used to
assess the genetic diversity from two hatchery stocks of freshwater prawn.
Samples were collected from two private hatcheries. Both stocks exhibited
significant variation across all five microsatellite loci. The average number
of alleles per locus was 6.4 and average expected heterozygosity at all loci
ranged from 0.81 to 0.82. FST per locus ranged from 0.03 to 0.11 and
the average FST value of 0.08 indicated that around 8 % of the total
genetic variation was explained by population differences, with the remaining
92 % corresponding to differences among individuals within population. Both the
hatchery stocks deviated significantly from Hardy-Weinberg expectations at all
the microsatellite loci.
Keywords:
Genetic diversity, microsatellite, Macrobrachiumrosenbergii,
Freshwater prawn.
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