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OJB©
Online Journal of Bioinformatics©
Onl J Bioinform©
Established 1995
ISSN 1443-2250
Volume 25 (2) :113-118,
2024.
Microsatellite alleles,
polymorphisms and heterozygosity in Tellichery goats (Capra
hircus).
Ramamoorthi
J, Sivaselvam
SN, Subramanian A, Kumarasamy P, Karthickeyan
SMK, Thangaraju P.
Department of Animal Genetics &
Breeding, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University,
Chennai, India
Ramamoorthi J, Sivaselvam SN,
Subramanian A, Kumarasamy P, Karthickeyan
SMK, Thangaraju P., Microsatellite alleles, polymorphisms and heterozygosity in Tellichery goats (Capra hircus), Onl J Bioinform., 25 (2) :113-118, 2024. Fifty
goat blood samples were taken from genetically unrelated Tellichery
goats with 5ml for extraction of genomic DNA. Twenty one microsatellite markers
were selected for molecular characterization by PCR for typing 10bp genotype single
homozygotes or double band heterozygotes. Allele frequencies were determined by
direct genotype count and expected compared with observed by c2 test. All 21 loci
were highly polymorphic with PIC values ranging 0.5785 to 0.8349. Significant
differences (P<0.01) were noticed between observed and expected genotypes at
14 loci with highest expected heterozygosity 0.8504 and lowest 0.6445.
KEYWORDS:
Goat, Genetic structure, Microsatellite markers.
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