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Online Journal of Bioinformatics

Established 1995

ISSN  1443-2250


Volume 24 (1):26-34, 2023.

In silico phylogenetics of functional proteins in entomo-pathogenic bacteria


Razia M1, Karthik Raja R1, Padmanaban K1, Chellapandi P2 and Sivaramakrishnan S1


1Department of Biotechnology, 2Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620024, Tamilnadu, India




Razia M, Karthik RR, Padmanaban K, Chellapandi P, Sivaramakrishnan S., In silico phylogenetics of functional proteins in entomo-pathogenic bacteria, Onl J Bioinform., 24 (1):26-34, 2023. Bacterial genes can synthetize polyketides. We describe In silico proteins of Photorhabdus luminescens subsp. laumondii TT01 by assigning functional relationship with polyketide synthases. PSI-BLAST was used to develop In silico proteins for homology to polyketides at genomic region 2247626-2249476 bp in chromosome 1. We found high similarity with ketoacyl synthase and acyl carrier protein. Keto-synthase-acyltransferase di-domain module 5 of Saccharopolyspora erythraea was suitable template for 3D model. ProFunc and Castp servers determined structure-function relationship and primary and secondary constructs resembled synthase homologs. Phylogenetic analysis of sequence and structure of β-polyketide synthase showed evolutionary relationship with soil and entero bacteria. We found horizontal gene transfer in P. luminescence genome suggesting abundance of polyketide genes in entomo pathogenic bacteria.


Keywords entomopathogenic nematodes, Heterorhabdus, Photorhabdus, ketoacyl synthase, phylogenetics, polyketides, gene annotation, functional assignment.