©2021-2032 All Rights Reserved. Online Journal of Bioinformatics. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to firstname.lastname@example.org publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Bioinformatics©
Volume 24 (1):26-34, 2023.
In silico phylogenetics of functional proteins in entomo-pathogenic bacteria
Razia M1, Karthik Raja R1, Padmanaban K1, Chellapandi P2 and Sivaramakrishnan S1
Razia M, Karthik RR, Padmanaban K, Chellapandi P, Sivaramakrishnan S., In silico phylogenetics of functional proteins in entomo-pathogenic bacteria, Onl J Bioinform., 24 (1):26-34, 2023. Bacterial genes can synthetize polyketides. We describe In silico proteins of Photorhabdus luminescens subsp. laumondii TT01 by assigning functional relationship with polyketide synthases. PSI-BLAST was used to develop In silico proteins for homology to polyketides at genomic region 2247626-2249476 bp in chromosome 1. We found high similarity with ketoacyl synthase and acyl carrier protein. Keto-synthase-acyltransferase di-domain module 5 of Saccharopolyspora erythraea was suitable template for 3D model. ProFunc and Castp servers determined structure-function relationship and primary and secondary constructs resembled synthase homologs. Phylogenetic analysis of sequence and structure of β-polyketide synthase showed evolutionary relationship with soil and entero bacteria. We found horizontal gene transfer in P. luminescence genome suggesting abundance of polyketide genes in entomo pathogenic bacteria.
Keywords entomopathogenic nematodes, Heterorhabdus, Photorhabdus, ketoacyl synthase, phylogenetics, polyketides, gene annotation, functional assignment.