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Online Journal of Bioinformatics ©
Volume 13(3):390-398, 2012.
Glycosyltransferase sequence informatics from different source organisms
Vivek Dhar Dwivedi¹, Amit Kumar Pandey², Sarad Kumar Mishra³*
¹Departments of Bioinformatics and ² Forest Pathology, Dehradun and ³Department of Biotechnology, DDU Gorakhpur University, Gorakhpur.
Dwivedi VD, Pandey AK, Mishra SK., Glycosyltransferase sequence informatics from different source organisms, Online J Bioinform., 13(3):390-398, 2012. Thirty full- length amino acid sequences of glycosyltransferase from fungi, plants and animals were collected and subjected to multiple sequence alignment (MSA), motif identification, domain identification, amino acid composition, and phylogenetic tree construction. MSA revealed that a single tryptophan residue was a conserved substitution in all plant and animal sequences profile, a single tryptophan residue was also conserved substitution in all plant and fungi sequences profile and no any conserved residue was found in animal and fungi sequences profile. Three major sequence clusters were constructed by phylogenetic analysis. Cluster I contained 7 species of plant, 5 of fungi, and 2 of animals, Cluster II had 3 species of plants, 3 of animals, and 2 of fungi, whereas Cluster III had 5 of animals and 3 of fungi. Amino acid composition result revealed that the average frequency of amino acid leucine was 9.84 percent which was high compared with other amino acids. The average frequency of cysteine was 1.79 which was low compared with analyzed species. Nine motifs which were unique for their groups were also identified.
Keywords: Glycosyltransferase. Phylogenetic analysis. Conserved regions. Motifs. Domains. Amino acid composition.