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Online Journal of Bioinformatics©
Volume 23 (1):1-18, 2022.
Interactions of microRNAs with Type 2 Diabetes mellitus in human genes
Manika Pal-Bhadra1, Neelima Arora2, Shinde Santosh P2, Paramita Ray1, Amit Kumar Banerjee 1, U.S.N Murty1, Utpal Bhadra2
1Centre for Chemical Biology and Biology Division, Indian Institute of Chemical Technology 2Functional Genomics and Gene Silencing Group, Centre for Cellular and Molecular Biology (CCMB), Hyderabad-500007, India.
Pal-Bhadra M, Arora N, Santosh PS, Ray P, Banerjee AK, Murty USN, Bhadra U., Interactions of microRNAs with Type 2 Diabetes mellitus in human genes , 23 (1):1-18, 2022. Complexity and multi-dimensional effects of Type II Diabetes mellitus (T2DM) are difficult to assess due to multiple genes and disease mechanism. Regulation of insulin secretion and glucose homeostasis is mediated by microRNAs so that knowledge of gene interactions that regulate T2DM is essential. Thirty genes are involved in two metabolic pathways in T2DM. We used miRanda (Version1.0) to predict target sites for microRNA expressed in pancreatic islets in T2DM associated genes. Target sites predicted by miRanda in 3’ UTR were compared with predictions of TargetScan and PicTar. Based on multiplicity and co-operativity, ten miRNAs targeting these genes were identified. Apart from predicting various miRNA target sites, several target site clusters marked as hot spots were also identified. Clustering of specific miRNA target sites on T2DM genes provides the clue for plausible role of these miRNAs in regulation of genes. We find that intersection of results recognized complete set of miRNAs targeting T2DM associated genes.
Keywords: Type 2 Diabetes mellitus, miRNA, miRANDA, , target site prediction.