MAIN


1996-2019. All Rights Reserved. Online Journal of Bioinformatics You may not store these pages in any form except for your own personal use. All other usage or distribute on is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the  before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or  re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page


OJBTM

 Online Journal of Bioinformatics 

 Volume 12(2):304-313, 2011.


Molecular docking ribavirin with RNA-dependent RNA polymerase crimean-congo hemorrhagic fever virus.

 

 Sitansu Kumar Verma*, Shivam Pratap Singh Yadav, Rhishika Singh, Ajay Kumar

 

Department of Biotechnology, Institute of Biomedical Education and Research, Mangalayatan University, Aligarh, India.

 

ABSTRACT

 

Kumar Verma S, Singh Yadav SP, Singh R, Kumar A, Molecular docking ribavirin with RNA-dependent RNA polymerase crimean-congo hemorrhagic fever virus, Onl J Bioinform., 12(2):304-313, 2011. Crimean-Congo hemorrhagic fever is a widespread tick borne viral disease affecting animals and humans. The viral membrane has RNA-dependent RNA polymerase (RDRP) which catalyses RNA synthesis in target cells. Inhibiting RDRP may thus prevent virus replication in host cells. The amino acid sequence of RDRP (Accession No: ACM78472.1) CCHF virus was retrieved from NCBI and a 3D model was built with Modeller9v8. The templates for RDRP protein were downloaded from PDB ID:3PHU. Homologous templates of RDRP exhibited sequence identity of 93.44% with the target. The RDRP model was then optimized with Modeller, validated through PROCHECK and submitted to PDB ID: PM0077454. The RDRP model predicted with AutoDock3.0.5., a ligand binding site for docking against the antiviral drug Ribavirin. The docked complexes were validated and enumerated using AutoDock to find suitable inhibitors.

 

Key words: Crimean-Congo hemorrhagic fever virus, RNA-dependent RNA Polymerase, Homology modeling, Ribavirin, Molecular docking.


MAIN

 

FULL-TEXT(SUBSCRIPTION OR PURCHASE TITLE $25USD)