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OJBTM
Online Journal of Bioinformatics ©
Volume 16(3): 318-326, 2015.
Model simulation phylogenetics
of erythropoietin expressed from cho cell line.
Manoj
Kumar Mettaa, Srinivasan Tantravahib*, Jayasimha
Rayalu Daddamc
a,bDepartment(s)
of Biotechnology, GIS, GITAM University, Gandhi Nagar, Rushikonda,
Visakhapatnam, Andhra Pradesh, cJawaharlal
Nehru Technological University, Anantapur,
Ananthapuram, Andhra Pradesh, India.
ABSTRACT
Mettaa MK, Tantravahib S, Daddam JR., Model
simulation phylogenetics of erythropoietin expressed
from cho cell line, Onl J Bioinform., 16(3): 318-326, 2015. Erythropoietin
(EPO), a ~30 kDa glycoprotein essential for
erythropoiesis, has a highly conserved amino acid sequence between monkey and
humans (~91% identity), and other animals. EPO binds and activates EPO
receptors (EPO-R) on the surface of erythroid precursor cells. We modeled a 3D structure of EPO generated with 1CN4 as
template with Modeller7v7 validated with Procheck and
Verify 3D graph. After energy minimization, the 3D structure was compared with
the template and 8 genes coding for EPO and its loci, were identified. Multiple
alignment and phylogenetic analysis showed that the EPO family proteins fall
into 4 branches. Mutations within the EPO domain region were identified.
KEY
WORDS: Erythropoietin; Modeling;
Molecular Dynamics; Validation; Phylogenetic analysis.
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