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Online Journal of Bioinformatics ©
Volume 16(3): 318-326, 2015.
Model simulation phylogenetics of erythropoietin expressed from cho cell line.
Manoj Kumar Mettaa, Srinivasan Tantravahib*, Jayasimha Rayalu Daddamc
a,bDepartment(s) of Biotechnology, GIS, GITAM University, Gandhi Nagar, Rushikonda, Visakhapatnam, Andhra Pradesh, cJawaharlal Nehru Technological University, Anantapur, Ananthapuram, Andhra Pradesh, India.
Mettaa MK, Tantravahib S, Daddam JR., Model simulation phylogenetics of erythropoietin expressed from cho cell line, Onl J Bioinform., 16(3): 318-326, 2015. Erythropoietin (EPO), a ~30 kDa glycoprotein essential for erythropoiesis, has a highly conserved amino acid sequence between monkey and humans (~91% identity), and other animals. EPO binds and activates EPO receptors (EPO-R) on the surface of erythroid precursor cells. We modeled a 3D structure of EPO generated with 1CN4 as template with Modeller7v7 validated with Procheck and Verify 3D graph. After energy minimization, the 3D structure was compared with the template and 8 genes coding for EPO and its loci, were identified. Multiple alignment and phylogenetic analysis showed that the EPO family proteins fall into 4 branches. Mutations within the EPO domain region were identified.
KEY WORDS: Erythropoietin; Modeling; Molecular Dynamics; Validation; Phylogenetic analysis.