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Online Journal of Bioinformatics©
Volume 19(3):234-239, 2018.
Subtractive genomics for membrane drug targets for antibiotic resistant Chlamydia trachomatis A/HAR-13.
Vijayakumari SM1*, Shivkumar M1, Biplab B2, Keshava M2, Jhinuk C2
1Department of Bioinformatics,KSW University, Bijapur, India. 2Department of Biotechnology, PES Institute of technology, Bangalore,India .
Vijayakumari SM, Shivkumar M, Biplab B, Keshava M, Jhinuk C., Subtractive genomics for membrane drug targets for antibiotic resistant Chlamydia trachomatis A/HAR-13, Onl J Bioinform., 19(3):234-239, 2018. A subtractive genomics approach was employed to identify metabolic pathways-related candidate drug and vaccine targets in Chlamydia trachomatis virulent strain A/HAR-13. A filter for drug proteome based on pre-set conditions to generate a set of membrane associated human-non homologous drug targets was used. Survival and segregation of Chlamydia trachomatis unique metabolic pathway proteins and structure generation of the membrane associated drug targets was validated. Five membrane associated drug targets participating in peptidoglycan biosynthesis; cell cycle caulobacter & lipopolysaccharide biosynthesis were identified and their 3D structures generated & validated. These putative drug targets identified can be used as a platform for screening against a battery of anti-microbial leads. Computational analysis of the result indicates that out of 919 proteins of Chlamydia trachomatis A/HAR-13; 314 were found to be essential after subjecting the non-homologous sequence to blastp against the DEG database. The essential gene of Chlamydia trachomatisA/HAR-13 is listed and analyzed for cellular localization and biochemical metabolic pathway analysis. The list of proteins predicted to have unique pathway is given and drug targets having unique pathogenic specific pathways localized in membrane were considered for further analysis since membrane bound proteins are easier to target and as they are surface proteins they are exposed to immune system as can be used an epitopes for vaccine design. A list of putative drug targets with its predicted function, localization, and pathway is given.
Keywords: subtractive genomics, drug targets, Chlamydia.