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OJBTM
Online Journal of Bioinformatics©
Volume 18(3):119-125, 2017.
Subtractive
genomics for membrane drug targets for antibiotic resistant Chlamydia trachomatis A/HAR-13.
Vijayakumari SM1*, Shivkumar
M1, Biplab B2,Keshava
M2, Jhinuk C2
1Department of Bioinformatics,KSW University, Bijapur, India. 2Department of Biotechnology,
PES Institute of technology, Bangalore, India .
ABSTRACT
Vijayakumari SM, Shivkumar M, Biplab B, Keshava M, Jhinuk C., Subtractive genomics for membrane drug targets
for antibiotic resistant Chlamydia trachomatis A/HAR-13, Onl
J bioinform., 18(3):119-125, 2017. A subtractive
genomics approach was employed to identify metabolic pathways-related candidate
drug and vaccine targets in Chlamydia
trachomatis virulent strain A/HAR-13. A filter for drug proteome based on pre-set
conditions to generate a set of membrane associated human-non homologous drug targets was used. Survival
and segregation of Chlamydia trachomatis
unique metabolic pathway proteins and structure generation of the membrane
associated drug targets was validated. Five membrane associated drug targets
participating in peptidoglycan biosynthesis; cell cycle caulobacter
& lipopolysaccharide biosynthesis were identified and their 3D structures
generated & validated. These putative drug targets identified can be used
as a platform for screening against a battery of anti-microbial leads. Computational analysis of the result
indicates that out of 919 proteins of Chlamydia
trachomatis A/HAR-13; 314 were found to be essential after subjecting the
non-homologous sequence to BLASTp against the DEG
database. The essential gene of Chlamydia
trachomatisA/HAR-13 is listed and analyzed for
cellular localization and biochemical metabolic pathway analysis. The list of
proteins predicted to have unique pathway is given and drug targets having
unique pathogenic specific pathways localized in membrane were considered for
further analysis since membrane bound proteins are easier to target and as they
are surface proteins they are exposed to immune system as can be used an
epitopes for vaccine design. A list of putative drug targets with its predicted
function, localization, and pathway is presented.
Keywords:
subtractive genomics, drug targets, Chlamydia
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