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Online Journal of Bioinformatics©
Volume 22 (2): 52-61, 2021.
Characterization and Sequencing of MT-COX1 gene from Gallus gallus.
Seyed Nader Albooshokeh1 , Mojtaba Tahmoorespur2 and Mohammad Reza Nassiry3
Department(s) 1Genetcs and Animal Breeding, 2Animal Genetics and Biotechnology, 3Animal Science, Ferdowsi University, Mashhad, Iran.
Albooshokeh SN, Tahmoorespur M, Nassiry MR., Characterization and Sequencing of MT-COX1 gene from chickens, Onl J Bioinform, 22 (2): ): 52-61, 2021. Two DNA fragments were amplified, cloned to pTZ57R/T vector and transformed into an E. coli - DH5α strain. Recombinant plasmids were then extracted from positive colonies and sequenced. The results of nucleotide sequence indicated maximum of similarity with the same sequence in the Gallus Gallus mitochondrial genome reference. Comparing these two sequences identified the presence of 3 mutations. Amino acid sequences of Khorasan’s native chicken had 99% similarity in 100% coverage with reference genome and other sequences with accession numbers YP272100.1, ADB06689.1, AHI87875.1, and YP272087.1. The protein BLAST query suggested the conserved domain cytochrome C, in a family which is part of a complex IV. Such mutations may be associated with the evolution of Khorasan's native chicken to adapt with environmental circumstances of its place of life.
KEY WORDS: Mitochondrial – Genome – Gallus Gallus