©1996-2019. All Rights Reserved. Online Journal of Bioinformatics . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJB publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Bioinformatics ©
Volume 14 (1): 56-65, 2013
Sequence informatics of carbonic anhydrase from different source organisms
Vivek Dhar Dwivedi, Shikha Arora, Jyoti Sharma, and Amit Pandey*
Forest Pathology Division, Forest Research Institute, Dehradun, India
Dwivedi VD, Arora S, Sharma J, Pandey A Sequence Informatics of Carbonic anhydrase Enzyme from Different Source organisms, Online J Bioinform., 14 (1): 56-65, 2013. Carbonic anhydrases are widely distributed among bacteria, fungi, plants and animals. Forty full length amino acid sequences of the enzyme were subjected to multiple sequence alignment (MSA), motif identification, domain identification, individual amino acid composition and phylogenetic analysis. MSA revealed that 1 alanine and glutamic acid residues were semi-conserved in all sequences while 1 proline and glycine residues were identically conserved in all sequences of fungi. Two major sequence clusters were obtained by phylogenetic analysis. One cluster contains 10 species of animals, 9 fungi, 2 bacteria and 1 plant, whereas another cluster contains 9 species of plants, 8 bacteria, and 1 fungus but no animal species were found in this cluster. Amino acid composition results revealed that the average frequency of alanine residue was 8.99% which was very high compared with the average frequency of other amino acids in all analyzed sequences. Fifteen amino acids Motif (QSPKAMVLACSDSRV) were found in all bacterial and plant sources. In addition, some motifs belonging to Pro_CA and Carb_anhydrase domain family unique to their groups, were also identified.
Keywords: Carbonic anhydrase, MSA, Phylogenetic analysis, Conserved regions, Motifs, Domains.
FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE $25USD)