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Online Journal of Bioinformatics ©
Volume 16(3): 303-317, 2015.
In silico analysis of arsenate reductase gene in biological systems
Sarita Tiwari1, Bijaya Ketan Sarangi1 Jeya Nasim2, Dinesh Yadav2
1National Environmental Engineering Research Institute, Council of Scientific and Industrial Research, Nehru Marg, Nagpur 2Department of Biotechnology, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh, India.
Tiwari S, Sarangi BK, Nasim J, Yadav D., In silico analysis of arsenate reductase gene in biological systems, Onl J Bioinform., 16(3): 303-317, 2015. ArsC cistron operon reduces arsenate As(V) to arsenite As(III), a primary step in arsenic (As) detoxification. It is presumed that this mechanism has evolved among prokaryotic bacteria and eukaryotic yeast and plants. 174 arsenate reductase (AR) protein sequences from 5 different species retrieved from NCBI were analyzed for similarity, evolution pathway, homology search, multiple sequence alignment, phylogenetic tree construction, motif and functional divergence. Conserved regions at different stretches of the sequences revealed the identity of AR and the phylogenetic tree showed 5 major cluster source organisms Escherichia coli, Staphylococcus aureus, Saccharomyces cerevisiae, Arabidopsis thaliana and Pteris vittata. Variability in the distribution of the six motifs was observed along with unique motif 4 and motif 6 exclusively for S. aureus and P. vittata respectively. The coefficient of functional divergence was found to be <1, indicating prevalence of site-specific selective constraints leading to functional evolution after diversification.
Keywords: Arsenic, arsenate reductase, evolution, phylogenetic relation