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OJBTM
Online Journal of
Bioinformatics ©
Volume 16(3): 303-317, 2015.
In silico analysis of arsenate reductase gene in biological systems
Sarita Tiwari1, Bijaya Ketan Sarangi1 Jeya Nasim2, Dinesh Yadav2
1National Environmental Engineering Research Institute, Council of
Scientific and Industrial Research, Nehru Marg, Nagpur 2Department of Biotechnology, DDU Gorakhpur University, Gorakhpur,
Uttar Pradesh, India.
ABSTRACT
Tiwari S, Sarangi BK, Nasim
J, Yadav D., In silico analysis of arsenate reductase gene in biological
systems, Onl J Bioinform., 16(3): 303-317, 2015. ArsC cistron operon reduces arsenate As(V) to arsenite As(III), a
primary step in arsenic (As) detoxification. It is presumed that this mechanism
has evolved among prokaryotic bacteria and eukaryotic yeast and plants. 174
arsenate reductase (AR) protein sequences from 5 different species retrieved
from NCBI were analyzed for similarity, evolution pathway, homology search,
multiple sequence alignment, phylogenetic tree construction, motif and
functional divergence. Conserved regions at different stretches of the
sequences revealed the identity of AR and the phylogenetic tree showed 5 major
cluster source organisms Escherichia coli, Staphylococcus aureus,
Saccharomyces cerevisiae, Arabidopsis thaliana and Pteris vittata.
Variability in the distribution of the six motifs was observed along with
unique motif 4 and motif 6 exclusively for S. aureus and P. vittata respectively. The coefficient of functional divergence
was found to be <1, indicating prevalence of site-specific selective
constraints leading to functional evolution after diversification.
Keywords: Arsenic, arsenate reductase, evolution,
phylogenetic relation
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