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Online Journal of Bioinformatics©
Volume 19(3):258-270, 2018.
Extraction of protein surface information from its aggregate.
Hrishikesh Mishra, Tapobrata Lahiri.
Division of Applied Sciences and Indo-Russian Center for Biotechnology, Indian Institute of Information Technology, Allahabad, India.
Mishra H, Lahiri T., Extraction of protein surface information from its aggregate, Onl J Bioinform., 19(3):258-270, 2018. Recent studies show that protein surface roughness profile could be used to identify small molecule attachment sites. Moreover, rough protein assembly carries a unique signature of its seed protein. Using these 2 charafcteristics we mapped a protocol for roughness profile of a protein from intensity level based multi fractal dimension (ILMFD) of its heat-denatured aggregates. The steps used were extraction of feature, ILMFD from microscopic images of heat denatured protein aggregate (HDPA), followed by mapping of ILMFD into Surface Roughness Index (SRI) through suitably chosen artificial neural network architecture. Our approach may determine a proteinís surface property without depending on its structure.
Key Words: Protein surface roughness, Intensity level based multifractal dimension, Heat denatured protein aggregate, Artificial Neural Network