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Online Journal of Bioinformatics©
Established 1995

ISSN  1443-2250


Volume 20(1):9-22, 2019.


 Homology based modeling of glial fibrillary acidic protein.

            

Sagarika Biswas, Saugata Roy, Rahul S. Mandal, H.R.Das

            

Institute of Genomics & Integrative Biology, Delhi University Campus, Mall Road,  Delhi – 110007. India

 

ABSTRACT

 

Biswas S, Roy S, Mandal RS, Das HR., Homology based modeling of glial fibrillary acidic protein, Onl J Bioinform., 20(1):9-22, 2019. Glial fibrillary acidic protein (GFAP) is an intermediate filament Type III protein. It contains three domains; the most conserved one is the rod domain. The specific DNA sequence for this region of the protein differs from the gene sequence of other filament proteins of Type III class. This feature makes this protein unique for studying the structure function relationship. In the present study, the 3D model of GFAP is generated using MODELLER 9v7. The sequence of GFAP was downloaded from NCBI (Accession No. AAB22581.1). Its stereo chemical property was analyzed by PROCHECK (RCSB Validation Server v11.1A) and the generated 3D model was visualized using Rasmol 3D viewer. This study will help in generating computer molecular models for which crystal structure is still not available.

 

Keywords: GFAP, Homology modeling, MODELLER, MolProbity, PROCHECK, Loop modeling.


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